1,48 All HIV-2 cases should be reported to the HIV surveillance program of the state or local. Geenius HIV1/2 HIV Western blot HIV confirmatory tests.Ĭopyright © 2014 Elsevier B.V. When used as a supplemental test, the results of the HIV-1 Western blot are more ambiguous than those of the HIV-1/HIV-2 antibody differentiation immunoassay >60 of individuals with HIV-2 are misclassified as having HIV-1 by the HIV-1 Western blot. The Bio-Rad Geenius HIV1/2 confirmatory system represents a reliable alternative to other confirmatory assays in HIV testing algorithms and provides clear improvement in quality management. In comparison with MP HIV BLOT 2.2, the Bio-Rad Geenius HIV1/2 assay was markedly time saving, allowed full traceability, automatic reading and interpretation. After excluding these last 13 samples, the specificity of Bio-Rad Geenius HIV1/2 assay reached 100%. Two out of the eight indeterminate samples by MP HIV BLOT 2.2 that were confirmed negative after follow-up were tested indeterminate and one invalid, the other five were negative. All five low-reactive samples by EIA, negative by HIV BLOT 2.2 were tested negative by Bio-Rad Geenius HIV1/2. All HIV negative serums by fourth-generation EIA were tested negative. After excluding early seroconversion samples, the sensitivity of Bio-Rad Geenius HIV1/2 assay reached 100%. Two out of the five HIV-2 reactive samples were tested positive HIV-2, two positive HIV-2 with HIV-1 cross-reaction and one HIV positive untypable. All HIV-1 non-B subtype samples were tested positive. Point of Care settings using rapid tests should consider anamnesis and patient risk assessment for an. Five out of 11 early seroconversion samples were tested positive, four negative and two indeterminate. Western blot may fail to detect an acute HIV-infection. WESTERN BLOT TEST RESULTS USING URINE SPECIMENS ARE NOT TO BE USED FOR. Objectives: We have evaluated the recently Conformité Européenne (CE)-marked Bio-Rad Geenius human immunodeficiency virus (HIV)1/2 as a rapid and simple alternative to western blot for confirmation of HIV screening results. The overall sensitivity for Bio-Rad Geenius HIV1/2 assay was 92%. FALSE POSITIVES MAY OCCUR MORE OFTEN WHEN TESTING URINE. The samples were tested according to the manufacturer's guidelines. We have evaluated the recently Conformité Européenne (CE)-marked Bio-Rad Geenius human immunodeficiency virus (HIV)1/2 as a rapid and simple alternative to western blot for confirmation of HIV screening results.Ī total of 160 serum samples were tested: 44 HIV-1 reactive samples by a fourth-generation Murex HIV Ag/Ab and/or Vidas HIV Duo Ultra, five HIV-2 reactive samples, 15 HIV-1 non-B subtype samples and 11 confirmed HIV-1 early seroconversion samples, 72 nonreactive samples, eight indeterminate samples by MP HIV BLOT 2.2 confirmed negative after follow-up and five low-reactive samples by enzyme immunoassay (EIA) negative by MP HIV BLOT 2.2. All 195 HIV-1-seroposi- tive subjects had detectable anti-HIV-1 antibodies in their saliva.
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